This chapter explores the development of Pedagogical Content Knowledge (PCK) among pre-service teachers, highlighting the challenges and innovations that shape their growth. Through detailed case studies, including the integration of technology, differentiated instruction, and culturally responsive teaching, the chapter examines effective strategies and the role of mentorship in enhancing PCK. It identifies common themes such as the importance of adapting teaching methods to diverse learner needs and the impact of reflective practice. Future directions emphasize the need for incorporating advanced technologies, data-driven instruction, and social-emotional learning into PCK frameworks. The chapter concludes by underscoring the significance of ongoing innovation and support in teacher preparation to improve educational outcomes.