This study delves into artificial intelligence (AI) literacy within Zambian academic libraries, focusing on librarians’ perceptions and applications of AI. The research aims to gauge the AI literacy level among Library and Information Science Professionals in Zambia, identify their awareness and knowledge of AI applications in libraries, and explore their perceptions regarding the advantages and challenges of implementing AI technologies in library services. Data from 82 diverse participants were gathered using purposive and convenience sampling methods. The findings indicate a solid understanding of AI fundamentals among Zambian librarians and positive attitudes towards AI’s potential benefits in library services. However, challenges such as the need for enhanced AI expertise, resistance to change, and budgetary constraints are acknowledged