Emotional intelligence (EI) is a critical driver of entrepreneurial success, influencing opportunity recognition, leadership, team dynamics, negotiation, resilience, and well-being. Traditional methods of assessing EI, including self-report and ability-based measures, are limited by subjectivity, cultural bias, and static evaluation. Recent advances in artificial intelligence (AI) offer innovative approaches for measuring EI through natural language processing, facial expression recognition, voice analysis, and physiological sensing, providing real-time, objective, and multimodal insights. AI-based assessments can enhance start-up team selection, investor evaluations, coaching, and stress management, while ethical considerations—such as algorithmic bias, privacy, and interpretive limitations—must be addressed. Integrating AI with EI theory enables dynamic, context-sensitive evaluation and development of emotional competencies, fostering more resilient and effective entrepreneurial ecosystems.